Lesson Plans for the Week of: 6-16-08

Class: Biotechnology Instructor: David Bruncke Room: 308 Period 5

Monday – Work day.

Tuesday – Work day.

Wednesday – Work day.

Thursday – Final (Plasmid test they have had to study from for a month)

Friday –

Assignments:

-Answer the pGLO questions on the handout that came with the lab

- Read Biology book 13.2. to learn about restriction enzymes (do mini-lab on p. 343, problem solving lab p. 347, Section assessment p. 348),

- solve plasmid cutting worksheet,

- make your own worksheet from NEB website (Go to NEB website and use the cutter tab to create a new worksheet for me. Choose a plasmid, cut it with at least 3 restriction enzymes (they should make at least 4 total cuts – more is great too), show the sizes of fragments when cut with each enzyme and each combination of enzymes. Make me a worksheet like the one I gave you and make an answer key for the cuts. Be sure to identify the plasmid and the enzymes.)]–

- find the 1 cutter enzymes that will cut the GFP gene from the pGLO plasmid. Predict the fragment size.

- GMO brochure

- PCR paper

-Restriction enzyme lab

*Grow the bacteria with the pGLO up and use the mini-prep kit to remove the plasmid.

*look at the enzymes we have and predict the fragment sizes when cut with 3 enzymes in every possible combination. Draw out lab directions. Cut the plasmid with the 3 enzymes and all combinations of them.

*make and run a gel with a ladder to see the fragment sizes.

-design the primers that would be needed to use PCR to make many copies of the GFP in a pGlo plasmid

-test over the information when the labs are done